Synthesis and characterization of the N-terminal acetylated 17-23 fragment of thymosin beta 4 identified in TB-500, a product suspected to possess doping potential

Characterization of Ac-LKKTETQ (TB-500's active fragment from thymosin beta-4) by HPLC/HRMS, establishing detection methods for anti-doping in competitive sport.

Abstract

The formulation TB-500 is suspected to be used as doping agent in sport.. This work describes the detection and the identification of the N-terminal acetylated 17-23 fragment of human thymosin beta 4 (Ac-LKKTETQ) in TB-500 by means of high-performance liquid chromatography/high resolution mass spectrometry using an Orbitrap Exactive benchtop mass spectrometer.. Ac-LKKTETQ was also synthesized by.

Key Findings

The formulation TB-500 is suspected to be used as doping agent in sport. | This work describes the detection and the identification of the N-terminal acetylated 17-23 fragment of human thymosin beta 4 (Ac-LKKTETQ) in TB-500 by means of high-performance liquid chromatography/... | Ac-LKKTETQ was also synthesized by solid-phase peptide synthesis, and an analytical strategy for detection in plasma and urine by high-performance liquid chromatography/low resolution triple-quadrupol...

Study Design

Research study